Purification and Properties of Orotic Acid-decarboxylating Enzymes from Calf Thymus
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Purification and properties of thymidylate synthetase from calf thymus.
The enzyme, thymidylate synthetase, catalyzes the methylation of deosyuridylate to thymidylate, with formaldehyde as the source of the methyl group, in the presence of tetrahydrofolate. Some of the properties of this enzyme system in crude soluble protein extracts of rat thymus have previously been reported (1). Reports have also appeared of this enzyme reaction in Escherichia cozi (2, 3). The ...
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The protein glutaredoxin, required for GSH-dependent ribonucleotide reduction, has been purified to homogeneity from calf thymus. The preparative method consisted of ammonium sulfate precipitation and three chromatography steps on DEAEkellulose, Sephadex G-50, and CM-Sepharose. Calf thymus glutaredoxin was assayed on the basis of its inherent GSH-disulfide transhydrogenase activity. Glutaredoxi...
متن کاملPurification and properties of ribonuclease H of calf thymus.
Ribonuclease H of calf thymus has been purified better than 3000-fold to yield an almost homogeneous preparation. The enzyme, which comprises about 0.03% of the total protein in the initial extract, is a slightly acidic protein (pI = 4.95) of molecular weight of about 64,000, possibly composed of subunits. The enzyme requires a metal ion for activation; the conditions for activation by Mg, Co, ...
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Kaplan, N. O., Everse, J. & Admiraal, J. (1968) Ann. N. Y. Acad. Sci. 151, 400-412 O'Carra, P. & Barry, S. (1972) FEBSLett. 21, 281-285 O'Carra, P. & Barry, S. (1974) Methods Enzymol. in the press O'Carra, P., Barry, S. & Corcoran, E. (1974) FEBS Lett. 43,163-168 Stambaugh, R. & Post, D. (1966) J. Biol. Chem. 241, 1462-1467 Wuntch, T., Vesell, E. S. & Chen, R. F. (1969) J. Biol. Chem. 244, 6100...
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Replication of polydeoxyadenylate and polydeoxythymidylate catalyzed by the high molecular weight DNA polymerase proceeds only in the presence of a complementary oligodeoxynucleotide. The oligodeoxynucleotide initiator chain must be greater than 6 nucleotides for poly(dA) replication and greater than 5 nucleotides for poly(dT) replication. The initiator oligonucleotide is incorporated into prod...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91164-6